Type: USC-F0.3-k0.3

Ultra-Short Cantilevers (for High-Speed AFM)

Logo
Cantilever Data Value Range*
Resonance Frequency 300 kHz 200 - 400 kHz
Force Constant 0.3 N/m 0.2 - 0.5 N/m
Length 20 µm 19 - 21 µm
Mean Width 10 µm 9.5 - 10.5 µm
Thickness 0.19 µm 0.17 - 0.21 µm

This AFM probe has alignment grooves on the back side of the support chip.

USC cantilever 3D view

USC cantilever 3D view More images

Product Description

NanoWorld® Ultra-Short Cantilevers (USC) for High-Speed AFM (HS-AFM) combine very small AFM cantilevers capable of resonating in the MHz regime and a very sharp and wear resistant AFM tip.
 
The AFM cantilever of the USC series is rectangular and made of a quartz-like material. A gold layer is deposited on both sides of the AFM cantilever in order to enhance the reflectance of the laser beam, but the AFM tip remains uncoated.

The wear resistant AFM tip has been developed together with nanotools GmbH and sustains high velocity scans over long distances. It is made of High Density Carbon/Diamond Like Carbon (HDC/DLC) material which is hard and wear resistant. It has a height of 2.5 microns and a radius of curvature smaller than 10 nm. The aspect ratio is in the order of 5 : 1 and the tilt compensation is 8° ensuring more symmetric AFM images.

The silicon support chip is of standard dimensions (1.6 mm x 3.4 mm x 0.3 mm). Additionally, it has etched and lowered corners in order to avoid contact between the support chip and the sample when scanning. Moreover it features alignment grooves on the back side of the silicon support chip which ensure replacement of the AFM probes without major adjustment of the laser beam when used in conjunction with the alignment chip.

The type USC-F0.3-k0.3 is mainly designed for High-Speed AFM applications in liquid but can also be used for applications in air (depending on the application).

Tip shape: Cone Shaped

Coating: Reflective Gold

Gold Reflex Coating

The gold reflex coating consists of a 30 nm thick gold layer deposited on both sides of the AFM cantilevers which enhances the reflectance of the laser beam. Furthermore it prevents light from interfering within the AFM cantilever. As the coating is almost stress-free the bending of the AFM cantilevers due to stress is less than 2 degrees

The AFM tip remains uncoated.

Order Codes

Order Code Quantity Data Sheet
USC-F0.3-k0.3-10 10 Nominal values

System limitations: Due to their small AFM cantilever sizes and their very high resonance frequencies USC probes currently cannot be used in all commercially available SPMs and AFMs. Only AFMs with a sufficiently small laser spot and electronics that are capable of dealing with high resonance frequencies of up to 5 MHz are able to work with the USC probes. If in doubt whether these AFM probes can be used in your AFM please check back with us or with your AFM manufacturer.

Product Screencast NanoWorld® Ultra-Short Cantilevers (USC) for High Speed Scanning

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Scientific publications mentioning use of this AFM probe


Matthew Chiriboga, Christopher M Green, Divita Mathur, David A Hastman, Joseph S Melinger, Remi Veneziano, Igor L Medintz and Sebastián A Díaz
Structural and optical variation of pseudoisocyanine aggregates nucleated on DNA substrates
Methods Appl. Fluoresc. (2023) 11 014003
DOI: 10.1088/2050-6120/acb2b4


Esra Oktay, Farhang Alem, Keziah Hernandez, Michael Girgis, Christopher Green, Divita Mathur, Igor L. Medintz, Aarthi Narayanan and Remi Veneziano
DNA origami presenting the receptor binding domain of SARS-CoV-2 elicit robust protective immune response
Communications Biology volume 6, Article number: 308 (2023)
DOI: https://doi.org/10.1038/s42003-023-04689-2


Matthew Chiriboga, Christopher M. Green, David A. Hastman, Divita Mathur, Qi Wei, Sebastían A. Díaz, Igor L. Medintz and Remi Veneziano
Rapid DNA origami nanostructure detection and classification using the YOLOv5 deep convolutional neural network
Nature Scientific Reports volume 12, Article number: 3871 (2022)
DOI: https://doi.org/10.1038/s41598-022-07759-3


Anna Mularski, Stine Lauritzen Sønder, Anne Sofie Busk Heitmann, Mayank Prakash Pandey, Himanshu Khandelia, Jesper Nylandsted and Adam Cohen Simonsen
Interplay of membrane crosslinking and curvature induction by annexins
Nature Scientific Reports volume 12, Article number: 22568 (2022)
DOI: https://doi.org/10.1038/s41598-022-26633-w


Katarzyna Wacnik, Vincenzo A. Rao, Xinyue Chen, Lucia Lafage, Manuel Pazos, Simon Booth, Waldemar Vollmer, Jamie K. Hobbs, Richard J. Lewis and Simon J. Foster
Penicillin-Binding Protein 1 (PBP1) of Staphylococcus aureus Has Multiple Essential Functions in Cell Division
American Society for Microbiology Journals, (2022) mBio, Vol. 13, No. 4
DOI: https://doi.org/10.1128/mbio.00669-22


Victor G. Gisbert and Ricardo Garcia
Accurate Wide-Modulus-Range Nanomechanical Mapping of Ultrathin Interfaces with Bimodal Atomic Force Microscopy
ACS Nano 2021, 15, 12, 20574–20581
DOI: https://doi.org/10.1021/acsnano.1c09178


Yang Xin, Guido Grundmeier and Adrian Keller
Adsorption of SARS-CoV-2 Spike Protein S1 at Oxide Surfaces Studied by High-Speed Atomic Force Microscopy
Advanced NanoBiomed Research, Volume 1, Issue 2, February 2021, 2000024
DOI: https://doi.org/10.1002/anbr.202000024


Victor G. Gisbert, Simone Benaglia, Manuel R. Uhlig, Roger Proksch, and Ricardo Garcia
High-Speed Nanomechanical Mapping of the Early Stages of Collagen Growth by Bimodal Force Microscopy
ACS Nano 2021, 15, 1, 1850–1857
DOI: https://doi.org/10.1021/acsnano.0c10159


Alena Khmelinskaia, Henri G. Franquelim, Renukka Yaadav, Eugene P. Petrov and Petra Schwille
Membrane-Mediated Self-Organization of Rod-Like DNA Origami on Supported Lipid Bilayers
Advanced Materials Interfaces, Volume 8, Issue 24, December 22, 2021, 2101094
DOI: https://doi.org/10.1002/admi.202101094


Christopher M. Green, David A. Hastman, Divita Mathur, Kimihiro Susumu, Eunkeu Oh, Igor L. Medintz, and Sebastián A. Díaz
Direct and Efficient Conjugation of Quantum Dots to DNA Nanostructures with Peptide-PNA
ACS Nano 2021, 15, 5, 9101–9110
DOI: https://doi.org/10.1021/acsnano.1c02296


Alexander Zika and Franziska Gröhn
Multiswitchable photoacid–hydroxyflavylium–polyelectrolyte nano-assemblies
Beilstein Journal of Organic Chemistry, 2021, 17, 166–185
DOI: https://doi.org/10.3762/bjoc.17.17


Anne Sofie Busk Heitmann, Ali Asghar Hakami Zanjani, Martin Berg Klenow, Anna Mularski,  Stine Lauritzen Sønder, Frederik Wendelboe Lund, Theresa Louise Boye, Catarina Dias, Poul Martin Bendix,  Adam Cohen Simonsen, Himanshu Khandelia and Jesper Nylandsted
Phenothiazines alter plasma membrane properties and sensitize cancer cells to injury by inhibiting annexin-mediated repair
Journal of Biological Chemistry (2021), Volume 297, ISSUE 2, 101012
DOI: https://doi.org/10.1016/j.jbc.2021.101012


Peter Reinholdt, Lütje E. Joensen, Daniel Petersen, Maria Szomek, Anna Mularski, Adam Cohen Simonsen, Jacob Kongsted and Daniel Wüstner
Photophysical and Structural Characterization of Intrinsically Fluorescent Sterol Aggregates
Journal of Physical Chemistry B 2021, 125, 22, 5838–5852
DOI: https://doi.org/10.1021/acs.jpcb.1c00628


Yang Xin, Charlotte Kielar, Siqi Zhu, Christoph Sikeler, Xiaodan Xu, Christin Möser, Guido Grundmeier, Tim Liedl, Amelie Heuer-Jungemann, David M. Smith and Adrian Keller
Cryopreservation of DNA Origami Nanostructures
Small (2020), Volume 16, Issue 13, 1905959
DOI: https://doi.org/10.1002/smll.201905959


Rachel Spokoini-Stern, Dimitar Stamov, Hadass Jessel, Lior Aharoni, Heiko Haschke, Jonathan Giron, Ron Unger, Eran Segal, Almogit Abu-Horowitz and Ido Bachelet
Visualizing the structure and motion of the long noncoding RNA HOTAIR
RNA 2020. 26: 629-636
DOI: 10.1261/rna.074633.120


Yang Xin, Salvador Martinez Rivadeneira, Guido Grundmeier, Mario Castro and Adrian Keller
Self-assembly of highly ordered DNA origami lattices at solid-liquid interfaces by controlling cation binding and exchange
NanoResearch 2020, 13(11): 3142–3150
DOI: https://doi.org/10.1007/s12274-020-2985-4


Todd H. Brintlinger, Susan Buckhout-White, Nabil D. Bassim, Divita Mathur, Anirban Samanta, Jeremy T. Robinson, Juan-Carlos Idrobo, Rhonda M. Stroud, Ellen R. Goldman and Mario G. Ancona
Chemical Mapping of Unstained DNA Origami Using STEM/EDS and Graphene Supports
ACS Applied Nano Materials 2020, 3, 2, 1123–1130
DOI: https://doi.org/10.1021/acsanm.9b02574


Alexander Zika, Sarah Bernhardt and Franziska Gröhn
Photoresponsive Photoacid-Macroion Nano-Assemblies
Polymers 2020, 12(8), 1746
DOI: https://doi.org/10.3390/polym12081746


Bohlen, Á. Cuartero-González, E. Pibiri, D. Ruhlandt, A. I. Fernández-Domínguez, P. Tinnefeld and G. P. Acuna
Plasmon-assisted Förster resonance energy transfer at the single-molecule level in the moderate quenching regime
Nanoscale, 2019,11, 7674-7681
DOI: https://doi.org/10.1039/C9NR01204D


Charlotte Kielar, Yang Xin, Xiaodan Xu, Siqi Zhu, Nelli Gorin, Guido Grundmeier, Christin Möser, David M. Smith and Adrian Keller
Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability
Molecules 2019, 24(14), 2577
DOI: https://doi.org/10.3390/molecules24142577


Matthijs Kol, Ben Williams, Henry Toombs-Ruane, Henri G Franquelim, Sergei Korneev, Christian Schroeer, Petra Schwille, Dirk Trauner, Joost CM Holthuis and James A Frank
Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides
eLife 8:e43230
DOI: https://doi.org/10.7554/eLife.43230.001


Henri G. Franquelim, Alena Khmelinskaia, Jean-Philippe Sobczak, Hendrik Dietz and Petra Schwille
Membrane sculpting by curved DNA origami scaffolds
Nature Communications volume 9, Article number: 811 (2018)
DOI: https://doi.org/10.1038/s41467-018-03198-9


Jihoon Shin, Junghoon Kim, Sung Ha Park and Tai Hwan Ha
Kinetic Trans-Assembly of DNA Nanostructures
ACS Nano 2018, 12, 9, 9423–9432
DOI: https://doi.org/10.1021/acsnano.8b04639


K.P. Sigdel, L.A. Wilt, B.P. Marsh, A.G. Roberts and G.M. King
The conformation and dynamics of P-glycoprotein in a lipid bilayer investigated by atomic force microscopy
Biochemical Pharmacology, Volume 156, October 2018, Pages 302-311
DOI: https://doi.org/10.1016/j.bcp.2018.08.017


Kenta Iijima, Junya Kobayashi and Yukihito Ishizaka
Structural alteration of DNA induced by viral protein R of HIV-1 triggers the DNA damage response
Retrovirology volume 15, Article number: 8 (2018)
DOI: https://doi.org/10.1186/s12977-018-0391-8


Alena Khmelinskaia, Henri G Franquelim, Eugene P Petrov and Petra Schwille
Effect of anchor positioning on binding and diffusion of elongated 3D DNA nanostructures on lipid membranes
Journal of Physics D: Applied Physics (2016) 49 194001
DOI: 10.1088/0022-3727/49/19/194001

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All data are subject to change without notice.

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