Type: Arrow™ TL2

Special Application AFM Cantilevers

Logo
Cantilever Data Value Range*
Resonance Frequency 6 kHz 3 - 14 kHz
Force Constant 0.03 N/m 0.004 - 0.54 N/m
Length 500 µm 495 - 505 µm
Mean Width 100 µm 95 - 105 µm
Thickness 1 µm 0.5 - 2.5 µm
ARROW-TL2

ARROW-TL2

Product Description

NanoWorld® Arrow™ TL2 probes are tipless AFM cantilevers for special applications. They can for example be used for attaching spheres and other objects to the free end of the AFM cantilever, or for functionalizing and sensing applications.

All SPM and AFM probes of the Arrow™ series are made from monolithic silicon which is highly doped to dissipate static charge and are chemically inert. The TL2 probes feature two rectangular AFM cantilevers having a triangular free end. The AFM cantilevers have a pitch of 250 µm.

The Arrow™ TL2 probes are optionally available with a sample facing side gold coating (Arrow™ TL2Au).

Tip shape: Tipless

Coating: none

Order Codes

Order Code Quantity Data Sheet
ARROW-TL2-50 50 Nominal values

NanoWorld® Arrow™ TL - Tipless AFM Cantilevers and AFM Cantilever Arrays Screencast

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Scientific publications mentioning use of this AFM probe


Catarina S. Lopes, Juliana Curty, Filomena A. Carvalho, A. Hernández-Machado, Koji Kinoshita, Nuno C. Santos and Rui D. M. Travasso
A mathematical model of fibrinogen-mediated erythrocyte–erythrocyte adhesion
nature Communications Biology volume 6, Article number: 192 (2023)
DOI: https://doi.org/10.1038/s42003-023-04560-4


Urszula Hohmann, Julian Cardinal von Widdern, Chalid Ghadban, Maria Cristina Lo Giudice, Grégoire Lemahieu, Elisabetta Ada Cavalcanti-Adam, Faramarz Dehghani and Tim Hohmann
Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension
Cells 2023, 12(1), 29
DOI: https://doi.org/10.3390/cells12010029


Francisco Drusso Martinez-Garcia, Tony Fischer, Alexander Hayn, Claudia Tanja Mierke, Janette Kay Burgess and Martin Conrad Harmsen
A Beginner’s Guide to the Characterization of Hydrogel Microarchitecture for Cellular Applications
Gels 2022, 8(9), 535
DOI: https://doi.org/10.3390/gels8090535


Patricia M. Silva, Inés V. da silva, Maria J. Sarmento, Itala C. silva, Filomena A. Carvalho, Graça Soveral and Nuno C. Santos
Aquaporin-3 and Aquaporin-5 Facilitate Migration and Cell–Cell Adhesion in Pancreatic Cancer by Modulating Cell Biomechanical Properties
Cells 2022, 11(8), 1308
DOI: https://doi.org/10.3390/cells11081308


Tim Hohmann, Urszula Hohmann, Mathias Dahlmann,  Dennis Kobelt, Ulrike Stein and Faramarz Dehghani
MACC1-Induced Collective Migration Is Promoted by Proliferation Rather Than Single Cell Biomechanics
Cancers 2022, 14(12), 2857
DOI: https://doi.org/10.3390/cancers14122857


Arnaud Blomme, Coralie Peter, Ernest Mui, Giovanny Rodriguez Blanco, Ning An, Louise M Mason, Lauren E Jamieson, Grace H McGregor, Sergio Lilla, Chara Ntala, Rachana Patel, Marc Thiry, Sonia H Y Kung, Marine Leclercq, Catriona A Ford, Linda K Rushworth, David J McGarry, Susan Mason, Peter Repiscak, Colin Nixon, Mark J Salji, Elke Markert, Gillian M MacKay, Jurre J Kamphorst, Duncan Graham, Karen Faulds, Ladan Fazli, Martin E Gleave, Edward Avezov, Joanne Edwards, Huabing Yin, David Sumpton, Karen Blyth, Pierre Close, Daniel J Murphy, Sara Zanivan and Hing Y Leung
THEM6‐mediated reprogramming of lipid metabolism supports treatment resistance in prostate cancer
EMBO Molecular Medicine (2022) 14: e14764
DOI: https://doi.org/10.15252/emmm.202114764


Patrick Schmidt, John Lajoie and Sanjeevi Sivasankar
Robust scan synchronized force-fluorescence imaging
Ultramicroscopy, Volume 221, February 2021, 113165
DOI: https://doi.org/10.1016/j.ultramic.2020.113165


Hyejeong Kim, Hannes Witt, Tabea A. Oswald and Marco Tarantola
Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting
ACS Applied Materials & Interfaces 2020, 12, 33516−33529
DOI: https://doi.org/10.1021/acsami.0c09166


Tim Hohmann, Urszula Hohmann, Marc R. Kolbe, Mathias Dahlmann, Dennis Kobelt, Ulrike Stein & Faramarz Dehghani
MACC1 driven alterations in cellular biomechanics facilitate cell motility in glioblastoma
Cell Communication and Signaling, (2020), Volume 18, article number 85
DOI: https://doi.org/10.1186/s12964-020-00566-1


Yan Nie, Xun Xu, Weiwei Wang, Nan Ma and Andreas Lendlein
Spheroid formation of human keratinocyte: Balancing between cell-substrate and cell-cell interaction
Clinical Hemorheology and Microcirculation. Vol. 76 (2020) 2, 329 -340
DOI: https://dx.doi.org/10.3233/CH-209217


Patrick D. Schmidt, Benjamin H. Reichert, John G. Lajoie, and Sanjeevi Sivasankar
Adaptive atomic force microscope
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124604 (13 February 2020)
DOI: https://doi.org/10.1117/12.2545261


Richa Karmakar, Christoph Schich, Nadine Kamprad, Vanessa Scheller, Edgar Gutierrez, Alex Groisman, Wouter-Jan Rappel and Marco Tarantola
Novel micropatterning technique reveals dependence of cell-substrate adhesion and migration of social amoebas on parental strain, development, and fluorescent markers
PLoS ONE 15(7): e0236171 (2020)
DOI: https://doi.org/10.1371/journal.pone.0236171


P. Černochová, L. Blahová, J. Medalová, D. Nečas, M. Michlíček, P. Kaushik, J. Přibyl, J. Bartošíková, A. Manakhov, L. Bačáková and L. Zajíčková
Cell type specific adhesion to surfaces functionalised by amine plasma polymers
nature Scientific Reports volume 10, Article number: 9357 (2020)
DOI: https://doi.org/10.1038/s41598-020-65889-y


Mark Skamrahl, Huw Colin-York, Liliana Barbieri and Marco Fritzsche
Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM–FRAP
nano micro small, Volume 15, Issue 40, October 1, 2019, 1902202
DOI: https://doi.org/10.1002/smll.201902202


Yuri Belotti, Serenella Tolomeo, Michael J. Conneely, Tianjun Huang, Stephen J. McKenna, Ghulam Nabi and David McGloin
High-Throughput, Time-Resolved Mechanical Phenotyping of Prostate Cancer Cells
nature Scientific Reports volume 9, Article number: 5742 (2019)
DOI: https://doi.org/10.1038/s41598-019-42008-0


Stefanie Puder, Tony Fischer and Claudia Tanja Mierke
The transmembrane protein fibrocystin/polyductin regulates cell mechanics and cell motility
Physical Biology, (2019) Volume 16, Number 6, 066006
DOI: https://doi.org/10.1088/1478-3975/ab39fa


Tom Kunschmann , Stefanie Puder, Tony Fischer, Anika Steffen, Klemens Rottner and Claudia Tanja Mierke
The Small GTPase Rac1 Increases Cell Surface Stiffness and Enhances 3D Migration Into Extracellular Matrices
nature Scientific Reports volume 9, Article number: 7675 (2019)
DOI: https://doi.org/10.1038/s41598-019-43975-0


Joseph H.R. Hetmanski, Henry de Belly, Ignacio Busnelli, Thomas Waring, Roshna V. Nair, Vanesa Sokleva, Oana Dobre, Angus Cameron, Nils Gauthier, Christophe Lamaze, Joe Swift, Aranzazu del Campo, Tobias Starborg, Tobias Zech, Jacky G. Goetz, Ewa K. Paluch, Jean-Marc Schwartz and Patrick T. Caswell
Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration
Developmental Cell (2019) 51, 460–475
DOI: https://doi.org/10.1016/j.devcel.2019.09.006


Mehmet Volkan Cakir, Uta Allenstein, Mareike Zink and Stefan G. Mayr
Early adhesion of cells to ferromagnetic shape memory alloys functionalized with plasma assembled biomolecules – a single cell force spectroscopy study
Materials & Design, Volume 158, 15 November 2018, Pages 19-27
DOI: https://doi.org/10.1016/j.matdes.2018.08.012


Yekaterina A. Miroshnikova, Huy Q. Le, David Schneider, Torsten Thalheim, Matthias Rübsam, Nadine Bremicker, Julien Polleux, Nadine Kamprad, Marco Tarantola, Irène Wang, Martial Balland, Carien M. Niessen, Joerg Galle and Sara A. Wickström
Adhesion forces and cortical tension couple cell proliferation and differentiation to drive epidermal stratification
nature cell biology, (2018), 20, pages 69–80
DOI: https://doi.org/10.1038/s41556-017-0005-z


Nadine Kamprad, Hannes Witt, Marcel Schröder,  Christian Titus Kreis, Oliver Bäumchen,  Andreas Janshoff  and  Marco Tarantola
Adhesion strategies of Dictyostelium discoideum – a force spectroscopy study
Nanoscale, 2018, 10, 22504-22519
DOI: https://doi.org/10.1039/C8NR07107A


Ana F. Guedes, Filomena A. Carvalho, Carlos Moreira, José B. Nogueira and Nuno C. Santos
Essential arterial hypertension patients present higher cell adhesion forces, contributing to fibrinogen-dependent cardiovascular risk
Nanoscale, (2017),9, 14897-14906
DOI: https://doi.org/10.1039/C7NR03891G


Tim Hohmann, Urszula Grabiec, Carolin Vogel, Chalid Ghadban, Stephan Ensminger, Matthias Bache, Dirk Vordermark and Faramarz Dehghani
The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties
International Journal of Molecular Sciences 2017, 18(9), 2001
DOI: https://doi.org/10.3390/ijms18092001


Tony Fischer, Nils Wilharm, Alexander Hayn and Claudia Tanja Mierke
Matrix and cellular mechanical properties are the driving factors for facilitating human cancer cell motility into 3D engineered matrices
Convergent Science Physical Oncology, 3 (2017) 044003
DOI: https://doi.org/10.1088/2057-1739/aa8bbb


Tom Kunschmann, Stefanie Puder, Tony Fischer, Jeremy Perez, Nils Wilharm and Claudia Tanja Mierke
Integrin-linked kinase regulates cellular mechanics facilitating the motility in 3D extracellular matrices
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1864, Issue 3, March 2017, Pages 580-593
DOI: https://doi.org/10.1016/j.bbamcr.2016.12.019


Helmar Leonhardt, Matthias Gerhardt, Nadine Höppner, Kirsten Krüger, Marco Tarantola and Carsten Beta
Cell-substrate impedance fluctuations of single amoeboid cells encode cell-shape and adhesion dynamics
Physical Review E (2016), 93, 012414
DOI: https://doi.org/10.1103/PhysRevE.93.012414


Thilo Baronskya, Aliaksandr Dzementseib, Marieelen Oelkersa, Juliane Melchertc, Tomas Pielerc and Andreas Janshoff
Reduction in E-cadherin expression fosters migration of Xenopus laevis primordial germ cells
Integrative Biology, Volume 8, Issue 3, March 2016, Pages 349–358
DOI: https://doi.org/10.1039/c5ib00291e


Chiann-Mun Chen, António M. A. Miranda, Gil Bub, Shankar Srinivas
Detecting cardiac contractile activity in the early mouse embryo using multiple modalities
Frontiers in Physiology, 07 January 2015, Sec. Cardiac Electrophysiology
DOI: https://doi.org/10.3389/fphys.2014.00508


Steve Pawlizak, Anatol W Fritsch, Steffen Grosser, Dave Ahrens, Tobias Thalheim, Stefanie Riedel, Tobias R Kießling, Linda Oswald, Mareike Zink and M Lisa Manning
Testing the differential adhesion hypothesis across the epithelial−mesenchymal transition
New Journal of Physics, Volume 17, August 2015, 083049
DOI: http://dx.doi.org/10.1088/1367-2630/17/8/083049


Andrew R Salmon, Matthew J Capener, Jeremy J Baumberg and Stephen R Elliot
Rapid microcantilever-thickness determination by optical interferometry
Measurement Science and Technology, (2014) 25, 015202 (7pp)
DOI: http://dx.doi.org/10.1088/0031-9155/57/5/1413


Marco Tarantola, Albert Bae, Danny Fuller, Eberhard Bodenschatz, Wouter-Jan Rappel and William F. Loomis
Cell Substratum Adhesion during Early Development of Dictyostelium discoideum
PLoS ONE 9(9): e106574 (2014)
DOI: https://doi.org/10.1371/journal.pone.0106574


Vicky Louise Morrison, Matthew MacPherson, Terhi Savinko, Hwee San Lek, Alan Prescott and Susanna Carola Fagerholm
The β2 integrin–kindlin-3 interaction is essential for T-cell homing but dispensable for T-cell activation in vivo
American Society of Hematology, blood, Volume 122, Issue 8, 22 August 2013, Pages 1428-1436
DOI: https://doi.org/10.1182/blood-2013-02-484998


Hwee San Lek, Vicky L. Morrison, Michael Conneely, Paul A. Campbell, David McGloin, Stefanie Kliche, Colin Watts, Alan Prescott, Susanna C. Fagerholm
The Spontaneously Adhesive Leukocyte Function-associated Antigen-1 (LFA-1) Integrin in Effector T Cells Mediates Rapid Actin- and Calmodulin-dependent Adhesion Strengthening to Ligand under Shear Flow
Journal of Biological Chemistry, Immunology, (2013) Volume 288, ISSUE 21, P14698-14708,
DOI: https://doi.org/10.1074/jbc.M112.430918


Martina A. Müller, Jan Opfe, Leonora Brunie, Lilli A. Volkhardt, Eva-Kathrin Sinner, David Boettiger, Alexander Bochen, Horst Kessler, Kay-Eberhard Gottschalk and Ute Reuning
The Glycophorin A Transmembrane Sequence within Integrin αvβ3 Creates a Non-Signaling Integrin with Low Basal Affinity That Is Strongly Adhesive under Force
Journal of Molecular Biology, Volume 425, Issue 16, 23 August 2013, Pages 2988-3006
DOI: http://dx.doi.org/10.1016/j.jmb.2013.05.020


K. Nieradka, H. Stegmann and T. Gotszalk
Focused ion beam milling and deposition techniques in validation of mass change value and position determination method for micro and nanomechanical sensors
Journal of Applied Physics (2012), 112, 114509
DOI: http://dx.doi.org/10.1063/1.4768715


K. Nieradka, G. Małozięć, D. Kopiec, P. Grabiec, P. Janus, A. Sierakowski and T. Gotszalk
Expanded beam deflection method for simultaneous measurement of displacement and vibrations of multiple microcantilevers
Review of Scientific Instruments, (2011), 82, 105112
DOI: https://doi.org/10.1063/1.3652977


Yen-I Chou, Hsu-Cheng Chiang, Chi-Chuan Wang
Study on Pd functionalization of microcantilever for hydrogen detection promotion
Sensors and Actuators B: Chemical, Volume 129, Issue 1, 29 January 2008, Pages 72-78
DOI: https://doi.org/10.1016/j.snb.2007.07.072

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